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Plex media server port 1230311/12/2022 ![]() In some embodiments, the MHC molecule is a class I MHC molecule. In some embodiments, the MHC multimer is a MHC tetramer. In some embodiments, the MHC molecule is a MHC multimer. In some embodiments, the cell is a T-cell. In some embodiments, the method further comprises using the peptide barcode and the sample barcode to identify the WIC molecule and the cell. In some embodiments, the method further comprises sequencing the nucleic acid molecule comprising the sample barcode sequence and the peptide barcode sequence to identify the sample barcode and the peptide barcode. In some embodiments, the method further comprises releasing the at least one nucleic acid barcode molecule from the additional particle. In some embodiments, the additional bead is a gel bead. In some embodiments, the additional particle is an additional bead. In some embodiments, the method further comprises prior to (c), releasing the peptide molecule and/or the at least one nucleic acid molecule from the particle In some embodiments, the method further comprises subsequent to (c), (1) providing an additional droplet or well comprising a cell having the derivative of the MHC molecule coupled thereto and an additional particle, which additional particle comprises at least one nucleic acid barcode molecule comprising a sample barcode sequence, and (2) using the at least one nucleic acid barcode molecule and the at least one nucleic acid molecule to generate another nucleic acid molecule that comprises the sample barcode sequence and the peptide barcode sequence, or a complement of the sample barcode sequence and the peptide barcode sequence. In some embodiments, the bead is a gel bead. In some embodiments, the particle is a bead. Provided herein are methods, compositions and systems for analyzing individual cells or a small population of cells, including the analysis and attribution of the analytes from and to these individual cells or cell populations.ĭisclosed herein, in some embodiments, is a method for processing a major histocompatibility complex (MHC) molecule, comprising: (a) providing a droplet or well comprising (i) the MHC molecule and (ii) a particle having at least one peptide molecule and at least one nucleic acid molecule comprising a peptide barcode sequence coupled thereto (b) attaching the at least one peptide molecule and the at least one nucleic acid molecule to the MHC molecule, to yield a derivative of the WIC molecule and (c) recovering the derivative of the WIC molecule from the droplet or well. Such cells include, but are not limited to, cancer cells, fetal cells, and immune cells involved in immune responses. Recognized herein is the need for methods, compositions, and systems for analyzing multiple analytes (e.g., genomic, epigenomic, transcriptome, and/or proteomic information) from individual cells or a small population of cells. Samples in partitions may be subjected to heating or cooling, or chemical reactions, such as to yield species that may be qualitatively or quantitatively processed. For example, such droplets may be fluidically isolated from other droplets, enabling accurate control of respective environments in the droplets.īiological samples in partitions may be subjected to various processes, such as chemical processes or physical processes. ![]() Droplets or wells may be employed to process biological samples in a manner that enables the biological samples to be partitioned and processed separately. There are various approaches for processing samples, such as polymerase chain reaction (PCR) and sequencing.īiological samples may be processed within various reaction environments, such as partitions. Biological samples may be processed, such as for detection of a disease (e.g., cancer) or identification of a particular species. BACKGROUNDĪ sample may be processed for various purposes, such as detection, identification, quantitation, and characterization of a type of moiety within the sample. The instant application contains a Sequence Listing which has been filed electronically in ASCII format and is hereby incorporated by reference in its entirety. 12, 2019, which claims the benefit of U.S. This application is a continuation of International Application No. ![]()
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